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Gold Bulletin

, Volume 45, Issue 4, pp 187–195

First Online: 20 September 2012

Abstract

In recent years, there has been considerable interest and research activity in using gold nanoparticle materials for biomedical applications including biomolecular detection, bioimaging, drug delivery, and photothermal therapy. In order to apply gold nanoparticles in the real biological world, we need to have a better understanding of the potential interactions between gold nanoparticle materials and biomolecules in vivo and in vitro. Here, we report the use of dynamic light scattering DLS for gold nanorods characterization and nanorod–protein interaction study. In the size distribution diagram, gold nanorods with certain aspect ratios exhibit two size distribution peaks, one with an average hydrodynamic diameter at 5–7 nm, and one at 70–80 nm. The small size peak is attributed to the rotational diffusion of the nanorods instead of an actual dimension of the nanorods. When proteins are adsorbed to the gold nanorods, the average particle size of the nanorods increases and the rotational diffusion-related size distribution peak also changes dramatically. We examined the interaction between four different proteins, bovine serum albumin, human serum albumin, immunoglobulin G, and immunoglobulin A IgA with four gold nanorods that have the same diameter but different aspect ratios. From this study, we found that protein adsorption to gold nanorods is strongly dependent on the aspect ratio of the nanorods, and varies significantly from protein to protein. The two serum albumin proteins caused nanorod aggregation upon interaction with the nanorods, while the two immunoglobulin proteins formed a stable protein corona on the nanorod surface without causing significant nanorod aggregation. This study demonstrates that DLS is a valuable tool for nanorod characterization. It reveals information complementary to molecular spectroscopic techniques on gold nanorod–protein interactions.

KeywordsGold nanoparticle Gold nanorod Protein interaction Dynamic light scattering Helin Liu and Nickisha Pierre-Pierre made equal contributions to this work.

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Autor: Helin Liu - Nickisha Pierre-Pierre - Qun Huo

Fuente: https://link.springer.com/



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