In vitro characterization of the antiviral activity of fucoidan from Cladosiphon okamuranus against Newcastle Disease VirusReportar como inadecuado




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Virology Journal

, 9:307

First Online: 12 December 2012Received: 25 May 2012Accepted: 07 December 2012

Abstract

BackgroundNewcastle Disease Virus NDV causes a serious infectious disease in birds that results in severe losses in the worldwide poultry industry. Despite vaccination, NDV outbreaks have increased the necessity of alternative prevention and control measures. Several recent studies focused on antiviral compounds obtained from natural resources. Many extracts from marine organisms have been isolated and tested for pharmacological purposes, and their antiviral activity has been demonstrated in vitro and in vivo. Fucoidan is a sulfated polysaccharide present in the cell wall matrix of brown algae that has been demonstrated to inhibit certain enveloped viruses with low toxicity. This study evaluated the potential antiviral activity and the mechanism of action of fucoidan from Cladosiphon okamuranus against NDV in the Vero cell line.

MethodsThe cytotoxicity of fucoidan was determined by the MTT assay. To study its antiviral activity, fusion and plaque-forming unit PFU inhibition assays were conducted. The mechanism of action was determined by time of addition, fusion inhibition, and penetration assays. The NDV vaccine strain La Sota was used in the fusion inhibition assays. PFU and Western blot experiments were performed using a wild-type lentogenic NDV strain.

ResultsFucoidan exhibited antiviral activity against NDV La Sota, with an obtained IS50 >2000. In time of addition studies, we observed viral inhibition in the early stages of infection 0–60 min post-infection. The inhibition of viral penetration experiments with a wild-type NDV strain supported this result, as these experiments demonstrated a 48% decrease in viral infection as well as reduced HN protein expression. Ribavirin, which was used as an antiviral control, exhibited lower antiviral activity than fucoidan and high toxicity at active doses. In the fusion assays, the number of syncytia was significantly reduced 70% inhibition when fucoidan was added before cleavage of the fusion protein, perhaps indicating a specific interaction between fucoidan and the F0 protein.

ConclusionThe results of this study suggest that fucoidan from C. okamuranus represents a potential low-toxicity antiviral compound for the poultry industry, and our findings provide a better understanding of the mode of action of sulfated polysaccharides.

KeywordsFucoidan NDV Antiviral Cladosiphon okamuranus Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-9-307 contains supplementary material, which is available to authorized users.

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Autor: Regina Elizondo-Gonzalez - L Elizabeth Cruz-Suarez - Denis Ricque-Marie - Edgar Mendoza-Gamboa - Cristina Rodriguez-Padilla

Fuente: https://link.springer.com/







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