RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomesReportar como inadecuado




RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Plant Molecular Biology

, 77:323

First Online: 05 August 2011Received: 10 May 2011Accepted: 16 July 2011

Abstract

Solanum lycopersicum and Solanum habrochaites f. typicum accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing RNA-seq to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S. lycopersicum genome resulted in the discovery of two additional sesquiterpene synthases expressed in trichomes. The sesquiterpene synthases from S. lycopersicum and S. habrochaites have high levels of protein identity. Several of them appeared to encode for non-functional proteins. Functional recombinant proteins produced germacrenes, β-caryophyllene-α-humulene, viridiflorene and valencene from E,E-farnesyl diphosphate. However, the activities of these enzymes do not completely explain the differences in sesquiterpene production between the two tomato plants. RT-qPCR confirmed high levels of expression of most of the S. lycopersicum sesquiterpene synthases in stem trichomes. In addition, one sesquiterpene synthase was induced by jasmonic acid, while another appeared to be slightly repressed by the treatment. Our data provide a foundation to study the evolution of terpene synthases in cultivated and wild tomato.

KeywordsSesquiterpene synthase Trichomes RNA-seq Wild and cultivated tomato Jasmonate Petra M. Bleeker and Eleni A. Spyropoulou contributed equally to this paper.

Electronic supplementary materialThe online version of this article doi:10.1007-s11103-011-9813-x contains supplementary material, which is available to authorized users.

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Autor: Petra M. Bleeker - Eleni A. Spyropoulou - Paul J. Diergaarde - Hanne Volpin - Michiel T. J. De Both - Philipp Zerbe -

Fuente: https://link.springer.com/



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