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Lipids in Health and Disease

, 10:133

First Online: 05 August 2011Received: 04 July 2011Accepted: 05 August 2011

Abstract

BackgroundAdvances in nanotechnology have identified promising candidates for many biological, biomedical and biomedicine applications. They are being increasingly exploited for medical uses and other industrial applications. The aim of the present study was to investigate the effects of administration of gold nanoparticles GNPs on inflammatory cells infiltration, central vein intima disruption, fatty change, and Kupffer cells hyperplasia in the hepatic tissue in an attempt to cover and understand the toxicity and the potential threat of their therapeutic and diagnostic use.

MethodsA total of 70 healthy male Wistar-Kyoto rats were exposed to GNPs received 50 or 100 μl of GNPs infusion of 10, 20 and 50 nm GNPs for 3 or 7 days. Animals were randomly divided into groups, 12 GNPs-treated rats groups and one control group NG. Groups 1, 2 and 3 received infusion of 50 μl GNPs of size 10 nm 3 or 7 days, size 20 nm 3 or 7 days and 50 nm 3 or 7 days, respectively; while groups 4, 5 and 6 received infusion of 100 μl GNPs of size 10 nm, size 20 nm and 50 nm, respectively.

ResultsIn comparison with respective control rats, exposure to GNPs doses has produced alterations in the hepatocytes, portal triads and sinusoids. The alterations in the hepatocytes were mainly vacuolar to hydropic degeneration, cytopasmic hyaline vacuolation, polymorphism, binucleation, karyopyknosis, karyolysis, karyorrhexis and necrosis. In addition, inflammatory cell infiltration, Kupffer cells hyperplasia, central veins intima disruption, hepatic strands dilatation and occasional fatty change together with a loss of normal architechiture of hepatic strands were also seen.

ConclusionsThe alterations induced by the administration of GNPs were size-dependent with smaller ones induced more affects and related with time exposure of GNPs. These alterations might be an indication of injured hepatocytes due to GNPs toxicity that became unable to deal with the accumulated residues resulting from metabolic and structural disturbances caused by these NPs. These histological alterations may suggest that GNPs interact with proteins and enzymes of the hepatic tissue interfering with the antioxidant defense mechanism and leading to reactive oxygen species ROS generation which in turn may induce stress in the hepatocytes to undergo necrosis.

Keywordsgold nanoparticles size hepatic tissue histology inflammatory fatty changes nanotoxicity rats Electronic supplementary materialThe online version of this article doi:10.1186-1476-511X-10-133 contains supplementary material, which is available to authorized users.

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Autor: Mohamed Anwar K Abdelhalim - Bashir M Jarrar

Fuente: https://link.springer.com/



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