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Virology Journal

, 8:390

First Online: 05 August 2011Received: 23 May 2011Accepted: 05 August 2011


Aichi virus is a positive-sense and single-stranded RNA virus, which demonstrated to be related to diarrhea of Children. In the present study, phylogenetic and recombination analysis based on the Aichi virus complete genomes available in GenBank reveal a mosaic genome sequence GenBank: FJ890523, of which the nt 261-852 region the nt position was based on the aligned sequence file shows close relationship with AB010145-Japan with 97.9% sequence identity, while the other genomic regions show close relationship with AY747174-German with 90.1% sequence identity. Our results will provide valuable hints for future research on Aichi virus diversity.

Aichi virus is a member of the Kobuvirus genus of the Picornaviridae family 1, 2 and belongs to a positive-sense and single-stranded RNA virus. Its presence in fecal specimens of children suffering from diarrhea has been demonstrated in several Asian countries 3, 4, 5, 6, in Brazil and German 7, in France 8 and in Tunisia 9. Some reports showed the high level of seroprevalence in adults 7, 10, suggesting the widespread exposure to Aichi virus during childhood.

The genome of Aichi virus contains 8,280 nucleotides and a polyA tail. The single large open reading frame nt 713-8014 according to the strain AB010145 encodes a polyprotein of 2,432 amino acids that is cleaved into the typical picornavirus structural proteins VP0, VP3, VP1, and nonstructural proteins 2A, 2B, 2C, 3A, 3B, 3C and 3D 2, 11. Based on the phylogenetic analysis of 519-bp sequences at the 3C-3D 3CD junction, Aichi viruses can be divided into two genotypes A and B with approximately 90% sequence homology 12. Although only six complete genomes of Aichi virus were deposited in GenBank at present, mosaic genomes can be found in strains from different countries.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-8-390 contains supplementary material, which is available to authorized users.

Xiaohong Han, Wen Zhang contributed equally to this work.

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Autor: Xiaohong Han - Wen Zhang - Yanjun Xue - Shihe Shao


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