Sequencing of bovine herpesvirus 4 v.test strain reveals important genome featuresReportar como inadecuado

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Virology Journal

, 8:406

First Online: 16 August 2011Received: 17 June 2011Accepted: 16 August 2011


BackgroundBovine herpesvirus 4 BoHV-4 is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi-s Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome BAC, the lack of a complete genome sequence for this strain limits its experimental use.

MethodsIn this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach.

ResultsThe long unique coding region LUR consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units prDNA. As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction prDNA-G differs from the other prDNA units prDNA-inner. Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions.

ConclusionsIn this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-8-406 contains supplementary material, which is available to authorized users.

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Autor: Leonor Palmeira - Bénédicte Machiels - Céline Lété - Alain Vanderplasschen - Laurent Gillet


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