LEAFY COTYLEDON1, FUSCA3 expression and auxin treatment in relation to somatic embryogenesis induction in ArabidopsisReportar como inadecuado




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Plant Growth Regulation

, Volume 65, Issue 1, pp 157–167

First Online: 29 March 2011Received: 06 September 2010Accepted: 12 March 2011

Abstract

The expression pattern of the LEC1 and FUS3 genes during somatic embryogenesis in Arabidopsis explants immature zygotic embryos induced in vitro was analysed, using Real-time quantitative PCR qRT-PCR. The analysis revealed differential expression of LEC1 but not FUS3 within a 30 day time course of somatic embryo development, and a significant auxin-dependent upregulation of LEC1 was found over the time course. In contrast to embryogenic culture, the level of LEC1 and FUS3 expression was noticeably lower in non-embryogenic callus of Col-0 and hormonal mutants cbp20 and axr4-1 with low SE-efficiency. In addition, the expression profile of LEC1 and FUS3 was followed in the embryogenic culture derived from 35S::LEC2-GR explants. A significant increase of LEC1 but not FUS3 activity was observed under LEC2 overexpression induced in auxin-treated explants. The work provides further experimental evidence on LEC gene involvement in the embryogenic response in Arabidopsis somatic cells, and also implicates LEC1 function in more advanced stages of SE culture in relation to somatic embryo differentiation and development.

KeywordsAuxin Embryogenic transition FUSCA3 Hormonal mutants LEAFY COTYLEDON1, LEC2 overexpression Abbreviations2,4-D2,4-dichlorophenoxyacetic acid

EInduction medium

IZEImmature zygotic embryo

SESomatic embryogenesis

ZEZygotic embryogenesis

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Autor: Agnieszka Ledwoń - Malgorzata D. Gaj

Fuente: https://link.springer.com/



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