MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changesReportar como inadecuado




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Lipids in Health and Disease

, 10:173

First Online: 30 September 2011Received: 31 August 2011Accepted: 30 September 2011

Abstract

BackgroundPolyunsaturated fatty acids PUFAs such as γ-linolenic acid GLA, arachidonic acid AA and docosahexaenoic acid DHA have cytotoxic action on glioma cells.

ResultsWe evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.

ConclusionsOur results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.

KeywordsPUFA micro RNA glioblastoma apoptosis Electronic supplementary materialThe online version of this article doi:10.1186-1476-511X-10-173 contains supplementary material, which is available to authorized users.

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Autor: Nóra Faragó - Liliána Z Fehér - Klára Kitajka - Undurti N Das - László G Puskás

Fuente: https://link.springer.com/



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