Chinese herbal extracts of Rubia cordifolia and Dianthus superbus suppress IgE production and prevent peanut-induced anaphylaxisReportar como inadecuado




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Chinese Medicine

, 6:35

First Online: 30 September 2011Received: 01 December 2010Accepted: 30 September 2011

Abstract

BackgroundPeanut allergy is characterized by increased levels of peanut-specific IgE in the serum of most patients. Thus, the most logical therapy would be to inhibit the IgE production by committed B-cells. This study aims to investigate the unreported anti-IgE effects of Chinese herbal extracts of Rubia cordifolia Qiancao and Dianthus superbus Qumai.

MethodsSeventy herbal extracts were tested for their ability to reduce IgE secretion by a human B-cell line. Those with the lowest inhibitory concentration 50 IC50 values were tested in a mouse model of peanut-anaphylaxis. Anaphylactic scores, body temperature, plasma histamine and peanut-specific-immunoglobulins were determined.

ResultsRubia cordifolia and Dianthus superbus inhibited the in vitro IgE production by a human B-cell line in a dose-dependent manner and the in vivo IgE production in a murine model of peanut allergy without affecting peanut-specific-IgG1 levels. After challenge, all mice in the sham groups developed anaphylactic reactions and increased plasma histamine levels. The extract-treated mice demonstrated significantly reduced peanut-triggered anaphylactic reactions and plasma histamine levels.

ConclusionThe extracts of Rubia cordifolia and Dianthus superbus inhibited the IgE production in vivo and in vitro as well as reduced anaphylactic reactions in peanut-allergic mice, suggesting potentials for allergy treatments.

AbbreviationsALTalanine aminotransferase

BUNblood urea nitrogen

CBCcomplete blood cells counts

CPEcrude peanut extract

DMSOdimethyl sulfoxide

FAHF2food allergy herbal formula 2

FBSfetal bovine serum

HbHemoglobin

i.g.intragastric

i.p.intraperitoneal

IC50inhibitory concentration 50

LD50lethal dose 50

MMCP1mouse mast-cell protease 1

MTT3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide

OIToral immunotherapy

PLTplatelets

PNApeanut allergy

QCQiancao

QMQumai

RBCred blood cells: SLIT: sublingual immunotherapy

WBCwhite blood cells.

Electronic supplementary materialThe online version of this article doi:10.1186-1749-8546-6-35 contains supplementary material, which is available to authorized users.

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Autor: Iván López-Expósito - Alexandra Castillo - Nan Yang - Banghao Liang - Xiu-Min Li

Fuente: https://link.springer.com/







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