Regeneration of Centella asiatica plants from non-embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plantsReportar como inadecuado




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Journal of Biological Engineering

, 5:13

First Online: 12 October 2011Received: 05 July 2011Accepted: 12 October 2011

Abstract

BackgroundThe threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years.

ResultsPresent study reveals that 6-benzyladenine BA either alone or in combination with 1-naphthalene acetic acid NAA supplemented in Murashige and Skoog MS medium at different concentrations produced good quality callus from leaf explants of C. asiatica. The calli produced on different plant growth regulators at different concentrations were mostly embryogenic and green. Highest shoot regeneration efficiency; 10 shoots per callus explant, from non-embryogenic callus was observed on 4.42 μM BA with 5.37 μM NAA. Best rooting response was observed at 5.37 and 10.74 μM NAA with 20 average number of roots per explant. Calli and regenerated plants extracts inhibited bacterial growth with mean zone of inhibition 9-13 mm diameter when tested against six bacterial strains using agar well diffusion method. Agar tube dilution method for antifungal assay showed 3.2-76% growth inhibition of Mucor species, Aspergillus fumigatus and Fusarium moliniformes.

ConclusionsThe present investigation reveals that non-embryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium. Furthermore, callus from leaf explant of C. asiatica can be a good source for production of antimicrobial compounds through bioreactor.

KeywordsAntimicrobial Centella asiatica organogenesis plant growth regulators List of abbreviationsBA6-benzyladenine

DMSOdimethyl sulfoxide

IBAindole 3-butyric acid

Kinkinetin

LSDLeast significant difference

MSMurashige and Skoog

NAAnephthalene acetic acid

Electronic supplementary materialThe online version of this article doi:10.1186-1754-1611-5-13 contains supplementary material, which is available to authorized users.

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Autor: Yamin Bibi - Muhammad Zia - Sobia Nisa - Darima Habib - Abdul Waheed - Fayyaz M Chaudhary

Fuente: https://link.springer.com/







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