Cerebellum-specific and age-dependent expression of an endogenous retrovirus with intact coding potentialReportar como inadecuado




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Retrovirology

, 8:82

First Online: 12 October 2011Received: 25 April 2011Accepted: 12 October 2011

Abstract

BackgroundEndogenous retroviruses ERVs, including murine leukemia virus MuLV type-ERVs MuLV-ERVs, are presumed to occupy ~10% of the mouse genome. In this study, following the identification of a full-length MuLV-ERV by in silico survey of the C57BL-6J mouse genome, its distribution in different mouse strains and expression characteristics were investigated.

ResultsApplication of a set of ERV mining protocols identified a MuLV-ERV locus with full coding potential on chromosome 8 named ERVmch8. It appears that ERVmch8 shares the same genomic locus with a replication-incompetent MuLV-ERV, called Emv2; however, it was not confirmed due to a lack of relevant annotation and Emv2 sequence information. The ERVmch8 sequence was more prevalent in laboratory strains compared to wild-derived strains. Among 16 different tissues of ~12 week-old female C57BL-6J mice, brain homogenate was the only tissue with evident expression of ERVmch8. Further ERVmch8 expression analysis in six different brain compartments and four peripheral neuronal tissues of C57BL-6J mice revealed no significant expression except for the cerebellum in which the ERVmch8 locus- low methylation status was unique compared to the other brain compartments. The ERVmch8 locus was found to be surrounded by genes associated with neuronal development and-or inflammation. Interestingly, cerebellum-specific ERVmch8 expression was age-dependent with almost no expression at 2 weeks and a plateau at 6 weeks.

ConclusionsThe ecotropic ERVmch8 locus on the C57BL-6J mouse genome was relatively undermethylated in the cerebellum, and its expression was cerebellum-specific and age-dependent.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4690-8-82 contains supplementary material, which is available to authorized users.

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Autor: Kang-Hoon Lee - Makoto Horiuchi - Takayuki Itoh - David G Greenhalgh - Kiho Cho

Fuente: https://link.springer.com/







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