Short term supplementation of dietary antioxidants selectively regulates the inflammatory responses during early cutaneous wound healing in diabetic miceReportar como inadecuado




Short term supplementation of dietary antioxidants selectively regulates the inflammatory responses during early cutaneous wound healing in diabetic mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Nutrition and Metabolism

, 8:80

First Online: 17 November 2011Received: 29 July 2011Accepted: 17 November 2011

Abstract

BackgroundDiabetic foot ulcers are serious complications for diabetic patients, yet the precise mechanism that underlines the treatment of these diabetic complications remains unclear. We hypothesized that dietary antioxidant supplementation with vitamin C, combined either with vitamin E or with vitamin E and NAC, improves delayed wound healing through modulation of blood glucose levels, oxidative stress, and inflammatory response.

MethodsDiabetes was induced by administration of alloxan monohydrate. Mice were divided into 4 groups; CON non-diabetic control mice fed AIN 93 G purified rodent diet, DM diabetic mice fed AIN 93 G purified rodent diet, VCE diabetic mice fed 0.5% vitamin C and 0.5% vitamin E supplemented diet, and Comb diabetic mice fed 0.5% vitamin C, 0.5% vitamin E, and 2.5% NAC supplemented diet. After 10 days of dietary antioxidant supplementation, cutaneous full-thickness excisional wounds were performed, and the rate of wound closure was examined. TBARS as lipid peroxidation products and vitamin E levels were measured in the liver. Expression levels of oxidative stress and inflammatory response related proteins were measured in the cutaneous wound site.

ResultsDietary antioxidant supplementation improved blood glucose levels and wound closure rate and increased liver vitamin E, but not liver TBARS levels in the diabetic mice as compared to those of the CON. In addition, dietary antioxidant supplementation modulated the expression levels of pIκBα, HO-1, CuZnSOD, iNOS and COX-2 proteins in the diabetic mice.

ConclusionsThese findings demonstrated that delayed wound healing is associated with an inflammatory response induced by hyperglycaemia, and suggests that dietary antioxidant supplementation may have beneficial effects on wound healing through selective modulation of blood glucose levels, oxidative stress, and inflammatory response.

KeywordsDiabetes antioxidant inflammation oxidative stress wound healing Electronic supplementary materialThe online version of this article doi:10.1186-1743-7075-8-80 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Na-Young Park - Yunsook Lim

Fuente: https://link.springer.com/







Documentos relacionados