Changes of microRNAs-192, 196a and 203 correlate with Barretts esophagus diagnosis and its progression compared to normal healthy individualsReportar como inadecuado




Changes of microRNAs-192, 196a and 203 correlate with Barretts esophagus diagnosis and its progression compared to normal healthy individuals - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Diagnostic Pathology

, 6:114

First Online: 17 November 2011Received: 21 September 2011Accepted: 17 November 2011

Abstract

BackgroundBarrett-s esophagus BE is a disease with a rising prevalence in western countries probably due to the unhealthy lifestyle. In significant number of cases it develops to esophageal adenocarcinoma. Two decades ago, important gene regulators microRNAs were discovered and their attendance in the process of malignant transformation was demonstrated e.g. miR-192, 196a, 203. Our aim was to select the patients with the increased risk of malignant transformation before the cancer develops.

Methods71 patients with BE disease were selected, slides from FFPE blocks were prepared, the lesions were microdissected and a qPCR relative expression analysis for selected microRNAs generally known to be connected with malignant transformation process was carried out.

ResultsWe demonstrated unequivocal statistically significant upregulation of two microRNAs miR-192, 196a and downregulation of miR-203 and positive miR-196a correlation with progression from intestinal metaplasia to adenocarcinoma compared to normal individuals.

ConclusionsWe hypothetize that there do exist changes of selected microRNAs which can undoubtedly distinguish the patients with BE from normal healthy individuals.

KeywordsmicroRNA Barrett-s esophagus real-time PCR List of abbreviations usedAFIautofluorescence

BEBarrett-s esophagus

cpthreshold point

DEPCdiethylpyrocarbamate

dNTPsdeoxynucleotide triphosphates

dTTPdeoxythymidine triphosphate

Eefficiency

EACesophageal adenocarcinoma

FFPEformalin fixed paraffin embedded

GERDgastroesophageal reflux disease

H-Ehematoxylin-eosin

ILinterleukin

LCMlaser capture microdissection

miRmicroRNA

mRNAmediator ribonucleic acid

NBInarrow band imaging

ntnucleotide

PASperiodic acid Schiff

PCRpolymerase chain reaction

PETpolyethylene terephthalate

qPCRquantitative polymerase chain reaction

RNAribonucleic acid

RTreverse transcription

UVultraviolet

Electronic supplementary materialThe online version of this article doi:10.1186-1746-1596-6-114 contains supplementary material, which is available to authorized users.

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Autor: Pavla Luzna - Jan Gregar - Ivo Uberall - Lenka Radova - Vlastimil Prochazka - Jiri EhrmannJr

Fuente: https://link.springer.com/







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