A short food frequency questionnaire to assess intake of seafood and n-3 supplements: validation with biomarkersReport as inadecuate

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Nutrition Journal

, 10:127

First Online: 19 November 2011Received: 30 June 2011Accepted: 19 November 2011


BackgroundSeafood intake is associated with beneficial effects for human health. Seafood provides a number of nutrients beyond the traditionally known long chain marine n-3 fatty acids EPA, DPA and DHA, such as protein, vitamin D, iodine, selenium and vitamin B12. Valid assessment of dietary seafood and n-3 supplement intakes are becoming increasingly crucial when giving recommendations to populations as seafood consumption is regarded as an important part of a healthy and balanced diet.

MethodsThe aim was to validate a short FFQ developed for assessment of dietary intake of seafood and n-3 supplements using the biomarkers marine n-3 fatty acids in erythrocytes and 25OHD in serum.

ResultsFifty-three healthy Norwegians aged 30-64 years with a mean BMI of 25 kg-m were compliant with the study protocol. 70% reported eating seafood for dinner one to two times per week, and 45% reported to eat seafood as spread, in salads or as snack meal three to five times or more per week. The FFQ correlated significantly with both the levels of marine n-3 fatty acids r = 0.73, p < 0.0001 and with 25OHD r = 0.37, p < 0.01. Mean level of marine n-3 and of 25OHD were 232 ± 65 μg-g erythrocytes and 73 ± 33 nmol-L serum, respectively.

ConclusionThe present short FFQ predicted strongly the levels of marine n-3 fatty acids in erythrocytes, and predicted fairly good the level of serum 25OHD and may therefore be a valid method for assessment of seafood and n-3 supplements intake among adults.

AbbreviationsEPAeicosapentaenoic acid, 20:5n-3

DPAdocosapentaenoic acid, 22:5n-3

DHAdocosahexaenoic acid, 22:6n-3

FFQfood frequency questionnaire

25OHD25-hydroxy Vitamin D

BMIbody mass index kg-m

LOQlimit of quantification

GLCgas liquid chromatographic

ICP-MSinductively coupled plasma mass-spectrometry.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2891-10-127 contains supplementary material, which is available to authorized users.

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Author: Lisbeth Dahl - Camilla A Mæland - Tormod Bjørkkjær

Source: https://link.springer.com/

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