Culture-independent molecular analysis of bacterial diversity in uranium-ore-mine waste-contaminated and non-contaminated sites from uranium minesReport as inadecuate




Culture-independent molecular analysis of bacterial diversity in uranium-ore-mine waste-contaminated and non-contaminated sites from uranium mines - Download this document for free, or read online. Document in PDF available to download.

3 Biotech

, Volume 1, Issue 4, pp 261–272

First Online: 03 November 2011Received: 02 June 2011Accepted: 18 October 2011

Abstract

Soil, water and sediment samples collected from in and around Jaduguda, Bagjata and Turamdih mines were analyzed for physicochemical parameters and cultured, and yet to be cultured microbial diversity. Culturable fraction of microbial community measured as Colony Forming Unit CFU on R2A medium revealed microbes between 10 and 10 CFU-g sample. Community DNA was extracted from all the samples; 16S rRNA gene amplified, cloned and subject to Amplified Ribosomal DNA Restriction Analysis. Clones representing each OTU were selected and sequenced. Sequence analyses revealed that non-contaminated samples were mostly represented by Acidobacteria, Bacteroidetes, Firmicutes and Proteobacteria β-, γ-, and-or δ-subdivisions along with less frequent phyla Nitrospira, Deferribacteres, Chloroflexi. In contrast, samples obtained from highly contaminated samples showed distinct abundance of β-,γ- and α-Proteobacteria along with Acidobacteria,Bacteroidetes and members of Firmicutes, Chloroflexi, Candidate division, Planctomycete, Cyanobacteria and Actinobacteria as minor groups. Our data represented the baseline information on bacterial community composition within non-contaminated samples which could potentially be useful for assessing the impact of metal and radionuclides contamination due to uranium mine activities.

KeywordsUranium mines 16S rRNA gene Bacterial community ARDRA  Download fulltext PDF



Author: Paltu Kumar Dhal - Ekramul Islam - Sufia K. Kazy - Pinaki Sar

Source: https://link.springer.com/







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