Evaluation of pullulan-functionalized doxorubicin nanoparticles for asialoglycoprotein receptor-mediated uptake in Hep G2 cell lineReport as inadecuate




Evaluation of pullulan-functionalized doxorubicin nanoparticles for asialoglycoprotein receptor-mediated uptake in Hep G2 cell line - Download this document for free, or read online. Document in PDF available to download.

Cancer Nanotechnology

, Volume 2, Issue 1–6, pp 49–55

First Online: 27 January 2011Received: 21 October 2010Accepted: 01 January 2011

Abstract

The present study discusses evaluation of pullulan-functionalized doxorubicin nanoparticles for asialoglycoprotein receptor-mediated uptake in the Hep G2 cell line. Doxorubicin hydrochloride DOX nanoparticles using polymers of different hydrophobic character, polyethylene sebacate hydrophobic and poly lactic-co-glycolic acid intermediate hydrophobicity with high entrapment efficiency and particle size were prepared by modified nanoprecipitation, using Gantrez AN 119 as complexing agent. Nanoparticles of Gantrez AN 119 were also prepared to represent a hydrophilic polymer. Cell uptake of DOX nanoparticles was found to be comparable to DOX solution irrespective of DOX concentration, nanoparticles size, and pullulan concentration. Furthermore, uptake of nanoparticles functionalized with or without pullulan prepared with polymers of different hydrophobic character revealed comparable uptake. Comparable uptake of DOX solution and DOX nanoparticles functionalized with or without pullulan suggest extracellular release of DOX as the mechanism of uptake from the nanoparticles. In vivo evaluation in hepatic cancer model is therefore essential to confirm the role of pullulan as asialoglycoprotein receptors ligand.

KeywordsDoxorubicin Asialoglycoprotein receptor Pullulan Hep G2 cells Polyethylene sebacate  Download fulltext PDF



Author: Swati A. Guhagarkar - Sharmila B. Majee - Abdul Samad - Padma V. Devarajan

Source: https://link.springer.com/







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