Identification of enzymes responsible for the reduction of geraniol to citronellolReport as inadecuate




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Natural Products and Bioprospecting

, Volume 1, Issue 3, pp 108–111

First Online: 13 December 2011Received: 17 November 2011Accepted: 30 November 2011

Abstract

The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K. Baker’s yeast was used in the bioreduction described above as a whole-cell biocatalyst. However, the enzyme responsible for the reduction of geraniol to citronellol is not yet known. Four old yellow enzyme OYE genes were cloned from yeast and plants, and expressed in Escherichia coli for a high level of recombinant proteins. The recombinant protein displayed a catalytic activity of converting geraniol to citronellol as a sole product verified by GC-MS analyses. The recombinant OYE2 intact cells were found to show 3.7 and 1.9-fold higher activity than that of yeast cells and the recombinant crude extracts, respectively. Compared to the recombinant fusion enzyme, the entrokinase-cleaved enzyme displayed nearly identical activity for geraniol reduction. To our knowledge, this is the first enzyme identified to catalyze the formation of citronellol from geraniol by reducing the allylic alcohol double bond, which is normally known as inactivating group for the old yellow enzymes. Open image in new window

Keywordsallylic alcohol citronellol geraniol old yellow enzyme reduction This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s13659-011-0032-6 and is accessible for authorized users.

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Author: Tian-Tian Yuan - Qian-Qian Chen - Pei-Ji Zhao - Ying Zeng - Xiao-Zhu Liu - Shan Lu

Source: https://link.springer.com/







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