Identification of N-acyl-l-homoserine lactones produced by non-pigmented Chromobacterium aquaticum CC-SEYA-1T and pigmented Chromobacterium subtsugae PRAA4-1TReport as inadecuate




Identification of N-acyl-l-homoserine lactones produced by non-pigmented Chromobacterium aquaticum CC-SEYA-1T and pigmented Chromobacterium subtsugae PRAA4-1T - Download this document for free, or read online. Document in PDF available to download.

3 Biotech

, Volume 1, Issue 4, pp 239–245

First Online: 14 October 2011Received: 28 April 2011Accepted: 30 September 2011

Abstract

Many members of the genus Chromobacterium produce violacein, a characteristic purple pigment which is induced by small diffusible N-acyl homoserine lactones AHL quorum-sensing molecules. In this study, the production of AHL of the non-pigmented C. aquaticum CC-SEYA-1 and the pigmented C. subtsugae PRAA4-1 were determined by using a CV026 biosensor assay. The profile of AHL was identified from the extracts of stationary phase cultures using gas chromatography–mass spectroscopy GC–MS and thin layer chromatography TLC. CV026 biosensor assay revealed that both the non-pigmented C. aquaticum CC-SEYA-1 and the pigmented C. subtsugae PRAA4-1 produced AHL molecules, which were identified, respectively, as N-octanoyl homoserine lactone OHL also known as C-8 homoserine lactone C8-HSL and N-hexanoyl homoserine lactone HHL also known as C-6 homoserine lactone C6-HSL. The pigment produced by C. subtsugae PRAA4-1 was similar to that of Chromobacterium violaceum ATCC12472 but no characteristic visible spectral peaks of the pigment were observed in the extracts of C. aquaticum CC-SEYA-1. In addition, C. aquaticum CC-SEYA-1 and C. subtsugae PRAA4-1 showed hemolytic activities.

KeywordsChromobacterium N-acyl homoserine lactones Violacein Quorum sensing  Download fulltext PDF



Author: P. D. Rekha - Chiu-Chung Young - A. B. Arun

Source: https://link.springer.com/







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