The cytokine-driven regulation of secretoglobins in normal human upper airway and their expression, particularly that of uteroglobin-related protein 1, in chronic rhinosinusitisReport as inadecuate




The cytokine-driven regulation of secretoglobins in normal human upper airway and their expression, particularly that of uteroglobin-related protein 1, in chronic rhinosinusitis - Download this document for free, or read online. Document in PDF available to download.

Respiratory Research

, 12:28

First Online: 01 December 2011Received: 15 October 2010Accepted: 08 March 2011

Abstract

BackgroundThe involvement of secretoglobins SCGBs other than SCGB1A1 Clara cell 10-kDa protein, CC10 in human airway diseases remains unexplored. Among those SCGBs, SCGB3A2 uteroglobin-related protein 1, UGRP1 is particularly interesting, given its structure and function similarities with SCGB1A1 CC10. The aim of this study was to investigate the expression regulation of SCGBs other than SCGB1A1 CC10 in human upper airway, and their potential involvement, particularly that of SCGB3A2 UGRP1, in chronic rhinosinusitis CRS with nasal polyps CRSwNP and without nasal polyps CRSsNP.

MethodsEight SCGB family members including SCGB3A2 UGRP1, SCGB1C1 ligand binding protein RYD5, SCGB1D1 lipophilin A, SCGB1D2 lipophilin B, SCGB1D4 interferon-γ inducible SCGB, SCGB2A1 mammaglobin 2, SCGB2A2 mammaglobin 1, and SCGB3A1 uteroglobin-related protein 2 were studied. The regulation of SCGBs mRNA expression in normal nasal mucosa by proinflammatory, Th1, and Th2 cytokines was studied through nasal explant culture. SCGBs mRNA expression levels in CRSsNP and CRSwNP patients and controls were compared. The mRNA levels were detected by means of quantitative reverse transcriptase-polymerase chain reaction. The protein expression of SCGB3A2 UGRP1 was analyzed using immunohistochemistry.

ResultsThe expression of SCGBs except SCGB1D2 lipophilin B could be found in upper airway and be differentially regulated by different cytokines. SCGB3A2 UGRP1 mRNA expression was induced by Th1 cytokine, but suppressed by proinflammatory and Th2 cytokines. SCGBs mRNA expression was altered in CRS; particularly, SCGB3A2 UGRP1 protein and mRNA expression was markedly decreased in both CRSsNP and CRSwNP and its protein levels inversely correlated with the number of total infiltrating cells, preoperative sinonasal CT scores, and postoperative endoscopy and symptom scores.

ConclusionSCGBs except SCGB1D2 lipophilin B are expressed in human upper airway and their expression can be differentially regulated by inflammatory cytokines. SCGBs mRNA expression is altered in CRS. Reduced production of UGRP1, which is likely due, at least in part, to a local cytokine environment, may contribute to the hyper-inflammation in CRS and correlates with response to surgery.

Electronic supplementary materialThe online version of this article doi:10.1186-1465-9921-12-28 contains supplementary material, which is available to authorized users.

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Author: Xiang Lu - Nan Wang - Xiao-Bo Long - Xue-Jun You - Yong-Hua Cui - Zheng Liu

Source: https://link.springer.com/







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