Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activityReport as inadecuate




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Annals of Microbiology

, Volume 61, Issue 4, pp 801–807

First Online: 22 January 2011Received: 22 June 2010Accepted: 05 January 2011

Abstract

Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder 1:1 w-v, 0.1% K2HPO4, and 0.05% MgSO4·7H2O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5–12, and 40–80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl DPPH as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine.

KeywordsChitinase Marine waste Alcaligenes faecalis Antioxidant  Download fulltext PDF



Author: Neelamegam Annamalai - Mayavan Veeramuthu Rajeswari - Shanmugam Vijayalakshmi - Thangavel Balasubramanian

Source: https://link.springer.com/







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