Localized Aurora B activity spatially controls non-kinetochore microtubules during spindle assemblyReport as inadecuate




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Chromosoma

, Volume 120, Issue 6, pp 599–607

First Online: 24 July 2011Received: 05 July 2011Revised: 11 July 2011Accepted: 13 July 2011

Abstract

Efficient spindle assembly involves the generation of spatial cues around chromosomes that locally stabilize microtubule MT plus-ends. In addition to the small GTPase Ran, there is evidence that Aurora B kinase might also generate a spatial cue around chromosomes but direct proof for this is still lacking. Here, we find that the Aurora B substrate MCAK localizes to MT plus-ends throughout the mitotic spindle, but its accumulation is strongly reduced on MT plus-ends near chromatin, suggesting that a signal emanating from chromosomes negatively regulates MCAK plus-end binding. Indeed, we show that Aurora B is the kinase responsible for producing this chromosome-derived signal. These results are the first to visualize spatially restricted Aurora B kinase activity around chromosomes on an endogenous substrate and explain how Aurora B could spatially control the dynamics of non-kinetochore MTs during spindle assembly.

Electronic supplementary materialThe online version of this article doi:10.1007-s00412-011-0334-9 contains supplementary material, which is available to authorized users.

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Author: Marvin E. Tanenbaum - René H. Medema

Source: https://link.springer.com/







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