Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8Report as inadecuate




Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8 - Download this document for free, or read online. Document in PDF available to download.

3 Biotech

, Volume 1, Issue 4, pp 255–259

First Online: 22 October 2011Received: 06 May 2011Accepted: 08 October 2011

Abstract

Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive optimum temperature 60 °C at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry.

KeywordsBioethanol Thermomyces lanuginosus Thermophilic fungi Xylanase Xylose  Download fulltext PDF



Author: Smriti Shrivastava - Pratyoosh Shukla - Kunal Mukhopadhyay

Source: https://link.springer.com/







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