Isolation of a novel thermostable dehydrochlorinase LinA from a soil metagenomeReport as inadecuate




Isolation of a novel thermostable dehydrochlorinase LinA from a soil metagenome - Download this document for free, or read online. Document in PDF available to download.

3 Biotech

, Volume 1, Issue 4, pp 193–198

First Online: 18 June 2011Received: 19 April 2011Accepted: 26 May 2011

Abstract

Hexachlorocyclohexane dehydrochlorinase LinA mediates first step of aerobic degradation of a chlorinated insecticide γ-hexachlorocyclohexane γ-HCH. In this study, we describe characterization of a novel variant LinA-type2 that is distinct from reported LinAs and is substantially more thermostable than archetypal LinA-UT26. LinA-type2 remains active even after 8 h of incubation at 45 °C, when nearly 50% activity of LinA-UT26 is lost after incubation for 60 min at the same temperature. Circular dichroism analysis revealed that secondary structures of LinA-UT26 and LinA-type2 are similar, but their Tm was 45 and 65 °C, respectively. Thermostability of LinA-type2 makes it suitable for bioreactors where allowance for higher temperatures can be of advantage.

KeywordsHexachlorocyclohexane Thermostability Dehydrochlorinase LinA Metagenome  Download fulltext PDF



Author: Ankit S. Macwan - Saleem Javed - Ashwani Kumar

Source: https://link.springer.com/







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