Expansion of CD4 CD25 helper T cells without regulatory function in smoking and COPDReport as inadecuate




Expansion of CD4 CD25 helper T cells without regulatory function in smoking and COPD - Download this document for free, or read online. Document in PDF available to download.

Respiratory Research

, 12:74

First Online: 01 December 2011Received: 22 December 2010Accepted: 08 June 2011

Abstract

BackgroundRegulatory T cells have been implicated in the pathogenesis of COPD by the increased expression of CD25 on helper T cells along with enhanced intracellular expression of FoxP3 and low-absent CD127 expression on the cell surface.

MethodRegulatory T cells were investigated in BALF from nine COPD subjects and compared to fourteen smokers with normal lung function and nine never-smokers.

ResultsIn smokers with normal lung function, the expression of CD25CD4 was increased, whereas the proportions of FoxP3 and CD127 were unchanged compared to never-smokers. Among CD4 cells expressing high levels of CD25, the proportion of FoxP3 cells was decreased and the percentage of CD127 was increased in smokers with normal lung function. CD4CD25 cells with low-absent CD127 expression were increased in smokers with normal lung function, but not in COPD, when compared to never smokers.

ConclusionThe reduction of FoxP3 expression in BALF from smokers with normal lung function indicates that the increase in CD25 expression is not associated with the expansion of regulatory T cells. Instead, the high CD127 and low FoxP3 expressions implicate a predominantly non-regulatory CD25 helper T-cell population in smokers and stable COPD. Therefore, we suggest a smoking-induced expansion of predominantly activated airway helper T cells that seem to persist after COPD development.

KeywordsBronchoalveolar lavage BAL CD25 CD127 FoxP3 lymphocyte subsets Electronic supplementary materialThe online version of this article doi:10.1186-1465-9921-12-74 contains supplementary material, which is available to authorized users.

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Author: Ester Roos-Engstrand - Jamshid Pourazar - Annelie F Behndig - Anders Bucht - Anders Blomberg

Source: https://link.springer.com/







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