Mometasone and desloratadine additive effect on eosinophil survival and cytokine secretion from epithelial cellsReport as inadecuate




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Respiratory Research

, 12:23

First Online: 01 December 2011Received: 06 October 2010Accepted: 27 February 2011

Abstract

BackgroundAlthough antihistamines and topical corticosteroids are used in combination to treat allergic rhinitis, their additive effect has not been yet demonstrated. The aim was investigate the antiinflammatory additive effect of mometasone and desloratadine on cytokine and sICAM-1 secretion by epithelial cells, and on eosinophil survival stimulated by human epithelial cells secretions from nasal mucosa and polyps.

MethodsEpithelial cells obtained from nasal mucosa or polyps were stimulated with 10% fetal bovine serum in presence of mometasone 10M-10M with-without desloratadine 10M. Cytokine and sICAM-1 concentrations in supernatants were measured by ELISA. Peripheral blood eosinophils were incubated during 4 days with epithelial cell secretions with 10M-10M and-or desloratadine 10M and survival assessed by Trypan blue. Results are expressed as percentage mean ± SEM compared to control.

ResultsFetal bovine serum stimulated IL-6, IL-8, GM-CSF and sICAM-1 secretion. In mucosa and polyp epithelial cells, mometasone inhibited this induced secretion while desloratadine inhibited IL-6 and IL-8. The combination of 10M desloratadine and 10M mometasone reduced IL-6 secretion 48 ± 11%, p < 0.05 greater extent than mometasone alone 68 ± 10% compared to control 100%. Epithelial cell secretions induced eosinophil survival from day 1 to 4, this effect being inhibited by mometasone. At day 4, the combination of mometasone 10M and desloratadine 10M provoked an increased inhibition of eosinophil survival induced by cell secretions 27 ± 5%, p < 0.01 than mometasone 44 ± 7% or desloratadine 46 ± 7% alone.

ConclusionsThese results suggest that the combination of desloratadine and mometasone furoate have a greater antinflammatory effect in an in vitro model of eosinophil inflammation than those drugs administered alone.

AbbreviationsARallergic rhinitis

CRSchronic rhinosinusitis

DLdesloratadine

DMSOdimethyl sulfoxide

FBSfetal bovine serum

GM-CSFgranulocyte-macrophage colony-stimulating factor

HECMhuman epithelial conditioned media

MFmometasone fuorate

NPnasal polyposis

PAFplatelet-activating factor

sICAM-1soluble intercellular adhesion molecule-1

TGF-βtransforming growth factor-β

TNF-αtumor necrosis factor-α

VEGFvascular endothelial growth factor.

Electronic supplementary materialThe online version of this article doi:10.1186-1465-9921-12-23 contains supplementary material, which is available to authorized users.

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Author: Joaquim Mullol - Francisco de Borja Callejas - Maria Asunción Martínez-Antón - Eva Méndez-Arancibia - Isam Alobid - Laur

Source: https://link.springer.com/







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