Ingraft chimerism in lung transplantation - a study in a porcine model of obliterative bronchiolitisReport as inadecuate

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Respiratory Research

, 12:56

First Online: 01 December 2011Received: 06 December 2010Accepted: 26 April 2011


BackgroundBronchial epithelium is a target of the alloimmune response in lung transplantation, and intact epithelium may protect allografts from rejection and obliterative bronchiolitis OB. Herein we study the influence of chimerism on bronchial epithelium and OB development in pigs.

MethodsA total of 54 immunosuppressed and unimmunosuppressed bronchial allografts were serially obtained 2-90 days after transplantation. Histology HandE was assessed and the fluorescence in situ hybridization FISH method for Y chromosomes using pig-specific DNA-label was used to detect recipient derived cells in graft epithelium and bronchial wall, and donor cell migration to recipient organs. Ingraft chimerism was studied by using male recipients with female donors, whereas donor cell migration to recipient organs was studied using female recipients with male donors.

ResultsEarly appearance of recipient-derived cells in the airway epithelium appeared predictive of epithelial destruction R = 0.610 - 0.671 and p < 0.05 and of obliteration of the bronchial lumen R = 0.698 and p < 0.01. All allografts with preserved epithelium showed epithelial chimerism throughout the follow-up. Antirejection medication did not prevent, but delayed the appearance of Y chromosome positive cells in the epithelium p < 0.05, or bronchial wall p < 0.05.

ConclusionsIn this study we demonstrate that early appearance of Y chromosomes in the airway epithelium predicts features characteristic of OB. Chimerism occurred in all allografts, including those without features of OB. Therefore we suggest that ingraft chimerism may be a mechanism involved in the repair of alloimmune-mediated tissue injury after transplantation.

AbbreviationsOBobliterative bronchiolitis

and FISHfluorescence in situ hybridization.

Electronic supplementary materialThe online version of this article doi:10.1186-1465-9921-12-56 contains supplementary material, which is available to authorized users.

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Author: Outi E Päiväniemi - Petra Musilova - Peter M Raivio - Paula K Maasilta - Hanni S Alho - Jiri Rubes - Kristiina Aittom


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