Optimization of xylanase production by Streptomyces sp. P12-137 using response surface methodology and central composite designReport as inadecuate




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Annals of Microbiology

, Volume 61, Issue 4, pp 773–779

First Online: 08 February 2011Received: 15 September 2010Accepted: 30 December 2010

Abstract

Response surface methodology and central composite design were used to optimize a biosynthesis medium for the production of xylanases by Streptomyces sp. P12-137 in submerged fermentation culture at pH 5.0, with wheat bran as substrate. The three variables involved in this research were the wheat bran, potassium nitrate and xylose concentrations. Statistical analysis of the results showed that, in the range studied, xylose and potassium nitrate concentrations had a significant effect on xylanase production. The optimized biosynthesis medium contained in %, w-v: wheat bran 1.0, KNO3 1.0, xylose 0.5. This medium resulted in a 3-fold increased level of the xylanase 27.77 UA-ml production compared to the initial level 8.30 UA-ml after 120 h of fermentation, whereas the value predicted by the quadratic model was 26.45 UA-ml.

KeywordXylanase Streptomyces Response surface methodology Optimization Central composite design  Download fulltext PDF



Author: Gigi Coman - Gabriela Bahrim

Source: https://link.springer.com/







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