Application of layered poly L-lactic acid cell free scaffold in a rabbit rotator cuff defect modelReport as inadecuate

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Sports Medicine, Arthroscopy, Rehabilitation, Therapy and Technology

, 3:29

First Online: 02 December 2011Received: 13 April 2011Accepted: 02 December 2011


BackgroundThis study evaluated the application of a layered cell free poly L-lactic acid PLLA scaffold to regenerate an infraspinatus tendon defect in a rabbit model. We hypothesized that PLLA scaffold without cultivated cells would lead to regeneration of tissue with mechanical properties similar to reattached infraspinatus without tendon defects.

MethodsLayered PLLA fabric with a smooth surface on one side and a pile-finished surface on the other side was used. Novel form of layered PLLA scaffold was created by superimposing 2 PLLA fabrics. Defects of the infraspinatus tendon were created in 32 rabbits and the PLLA scaffolds were transplanted, four rabbits were used as normal control. Contralateral infraspinatus tendons were reattached to humeral head without scaffold implantation. Histological and mechanical evaluations were performed at 4, 8, and 16 weeks after operation.

ResultsAt 4 weeks postoperatively, cell migration was observed in the interstice of the PLLA fibers. Regenerated tissue was directly connected to the bone composed mainly of type III collagen, at 16 weeks postoperatively. The ultimate failure load increased in a time-dependent manner and no statistical difference was seen between normal infraspinatus tendon and scaffold group at 8 and 16 weeks postoperatively. There were no differences between scaffold group and reattach group at each time of point. The stiffness did not improve significantly in both groups.

ConclusionsA novel form of layered PLLA scaffold has the potential to induce cell migration into the scaffold and to bridge the tendon defect with mechanical properties similar to reattached infraspinatus tendon model.

Electronic supplementary materialThe online version of this article doi:10.1186-1758-2555-3-29 contains supplementary material, which is available to authorized users.

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Author: Atsuyuki Inui - Takeshi Kokubu - Hiroyuki Fujioka - Issei Nagura - Ryosuke Sakata - Hanako Nishimoto - Masaru Kotera - Taka


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