Endurance exercise is a leptin signaling mimetic in hypothalamus of Wistar ratsReport as inadecuate

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Lipids in Health and Disease

, 10:225

First Online: 02 December 2011Received: 24 November 2011Accepted: 02 December 2011


BackgroundEndurance exercise is known to promote a substantial effect on the energy balance in rats and humans. However, little is known about the exact mechanisms for the appetite-suppressive effects of endurance exercise. We hypothesized that endurance training might activate signaling cascades in the hypothalamus known to be involved in leptin signaling.

Methods16 male Wistar rats were randomly assigned to two groups: sedentary n = 8 and exercise groups n = 8. Animals in the exercise group started treadmill running at 30 m-min, 0% grade, for 1 min-bout. Running time was gradually increased by 2 min-bout every day. The training plan was one bout per day during initial two weeks, and two bouts per day during 3rd-9th week. At the end of nine-week experiment, blood was analyzed for low-density lipoprotein cholesterol LDL-C, triglyceride TG, total cholesterol TC, free fatty acid FFA, interleukin IL-6, and leptin in both groups. Activations of janus kinase 2-signaling transducer and activator of transcription 3 JAK2-STAT3, protein kinase B Akt, extracellular regulated kninase ERKs, and suppressor of cytokine signaling 3 SOCS3 in hypothalamus were measured in the end of nine weeks of exercise protocol.

ResultsNine-week endurance exercise induced lower concentrations of LDL-C, TG, TC, FFA, and leptin in rats P < 0.05 or P < 0.01. Nine-week endurance exercise significantly increased the circulating IL-6 concentration compared with sedentary group 239.6 ± 37.2 pg-ml vs. 151.8 ± 31.5 pg-ml, P < 0.01. Exercise rats showed significant increases in JAK2, STAT3, Akt, ERKs, and SOCS3 phosphorylations compared with sedentary rats P < 0.01.

ConclusionThe data suggest that endurance exercise is a leptin signaling mimetic in hypothalamus of Wistar rats.

KeywordsTREADMILL JAK2 STAT3 Akt ERKs SOCS3 IL-6 Electronic supplementary materialThe online version of this article doi:10.1186-1476-511X-10-225 contains supplementary material, which is available to authorized users.

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Author: Jiexiu Zhao - Ye Tian - Jincheng Xu - Dongsen Liu - Xiaofang Wang - Binxiu Zhao

Source: https://link.springer.com/

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