Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in ChinaReport as inadecuate

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Virology Journal

, 8:557

First Online: 22 December 2011Received: 21 July 2011Accepted: 22 December 2011


BackgroundBovine adenovirus type 3 BAV-3 belongs to the Mastadenovirus genus of the family Adenoviridae and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined.

ResultsThe size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the Mastadenovirus genus of the family Adenoviridae.

ConclusionsThis is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family Adenoviridae on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological investigations for BVA-3 infection might be carried out in cattle in China. This report will be a good beginning for further studies on BAV-3 in China.

KeywordsBovine adenovirus type 3 Cattle Complete genome DNA binding protein List of AbbreviationsBAVbovine adenovirus

BAV-3bovine adenovirus type 3

bpbase pair

BRDCbovine respiratory disease complex

CDScoding sequence

CPEcytopathic effect

DBPDNA binding protein

FITCfluorescein isothiocyanate

ITRinverted terminal repeat

MDBKMadin-Darby bovine kidney

MEMminimum essential medium


ORFopen reading frame

PBSphosphate buffered saline

PCRpolymerase chain reaction.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-8-557 contains supplementary material, which is available to authorized users.

Yuan-Mao Zhu, Zuo Yu contributed equally to this work.

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Author: Yuan-Mao Zhu - Zuo Yu - Hong Cai - Yu-Ran Gao - Xiu-Mei Dong - Zhao-Li Li - Hong-Fei Shi - Qing-Feng Meng - Chuang Lu - F


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