Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirsReportar como inadecuado




Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

AMB Express

, 1:47

First Online: 23 December 2011Received: 20 October 2011Accepted: 23 December 2011

Abstract

Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortium, refining our previous observations of aerobic degradation. The aerobic consortium depleted, in decreasing order, hydrocarbons > hopanes > steranes > tricyclic terpanes while the anaerobic consortium depleted hydrocarbons > steranes > hopanes > tricyclic terpanes. The oxygen content of the mixed consortia was measured from time to time revealing alternating periods of microaerobicity O2 ~0.8 mg.L and of aerobicity O2~6.0 mg.L. In this experiment, the petroleum biodegradation changed from time to time, alternating periods of biodegradation similar to the aerobic process and periods of biodegradation similar to the anaerobic process. The consortia showed preferences for metabolizing hydrocarbons > hopanes > steranes > tricyclic terpanes during a 90-day period, after which this trend changed and steranes were more biodegraded than hopanes. The analysis of aerobic oil degrading microbiota by the 16S rRNA gene clone library detected the presence of Bacillus, Brevibacterium, Mesorhizobium and Achromobacter, and the analysis of the anaerobic oil degrading microbiota using the same technique detected the presence of Bacillus and Acinetobacter facultative strains. In the mixed consortia Stenotrophomonas, Brevibacterium, Bacillus, Rhizobium, Achromobacter and 5% uncultured bacteria were detected. This is certainly a new contribution to the study of reservoir biodegradation processes, combining two of the more important accepted hypotheses.

KeywordsPetroleum biodegradation oxic environment anoxic environment 16S rRNA gene petroleum biomarkers. Electronic supplementary materialThe online version of this article doi:10.1186-2191-0855-1-47 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Georgiana F da Cruz - Suzan P de Vasconcellos - Célio FF Angolini - Bruna M Dellagnezze - Isabel NS Garcia - Valéria 

Fuente: https://link.springer.com/







Documentos relacionados