High fat diet enhances cardiac abnormalities in SHR rats: Protective role of heme oxygenase-adiponectin axisReport as inadecuate

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Diabetology and Metabolic Syndrome

, 3:37

First Online: 23 December 2011Received: 11 October 2011Accepted: 23 December 2011


BackgroundHigh dietary fat intake is a major risk factor for development of cardiovascular and metabolic dysfunction including obesity, cardiomyopathy and hypertension.

MethodsThe present study was designed to examine effect of high fat HF diet on cardio-vascular structure and function in spontaneously hypertensive rats SHR, fed HF diet for 15 weeks, a phenotype designed to mimic metabolic syndrome.

ResultsDevelopment of metabolic syndrome like phenotype was confirmed using parameters, including body weight, total cholesterol and blood pressure levels. High fat diet impaired vascular relaxation by acetylcholine and exacerbated cardiac dysfunction in SHRs as evidenced by lower left ventricular function, and higher coronary resistance CR as compared to controls p < 0.05. The histological examination revealed significant myocardial and peri-vascular fibrosis in hearts from SHRs on HF diet. This cardiac dysfunction was associated with increased levels of inflammatory cytokines, COX-2, NOX-2, TxB2 expression and increase in superoxide O2 levels in SHR fed a HF diet p < 0.05. HO-1 induction via cobalt-protoporphyrin CoPP,3 mg-kg, in HF fed rats, not only improved cardiac performance parameters, but also prevented myocardial and perivascular fibrosis. These effects of CoPP were accompanied by enhanced levels of cardiac adiponectin levels, pAMPK, peNOS and iNOS expression; otherwise significantly attenuated p < 0.05 in HF fed SHRs. Prevention of such beneficial effects of CoPP by the concurrent administration of the HO inhibitor stannic mesoporphyrin SnMP corroborates the role of HO system in mediating such effects.

ConclusionIn conclusion, this novel study demonstrates that up-regulation of HO-1 improves cardiac and vascular dysfunction by blunting oxidative stress, COX-2 levels and increasing adiponectin levels in hypertensive rats on HF diet.

Keywordsheme oxygenase adiponectin high fat diet COX-2 oxidative stress Electronic supplementary materialThe online version of this article doi:10.1186-1758-5996-3-37 contains supplementary material, which is available to authorized users.

Jian Cao, Komal Sodhi contributed equally to this work.

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Author: Jian Cao - Komal Sodhi - Nitin Puri - Sumit R Monu - Rita Rezzani - Nader G Abraham

Source: https://link.springer.com/

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