A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliananatural variationReportar como inadecuado




A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliananatural variation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Plant Biology

, 10:158

First Online: 03 August 2010Received: 25 March 2010Accepted: 03 August 2010

Abstract

BackgroundThe proteasome subunit RPT5, which is essential for gametophyte development, is encoded by two genes in Arabidopsis thaliana; RPT5a and RPT5b. We showed previously that RPT5a and RPT5b are fully redundant in the Columbia Col-0 accession, whereas in the Wassilewskia accession Ws-4, RPT5b does not complement the effect of a strong rpt5a mutation in the male gametophyte, and only partially complements rpt5a mutation in the sporophyte. RPT5b and RPT5b differ by only two SNPs, one located in the promoter and the other in the seventh intron of the gene.

ResultsBy exploiting natural variation at RPT5b we determined that the SNP located in RPT5b intron seven, rather than the promoter SNP, is the sole basis of this lack of redundancy. In Ws-4 this SNP is predicted to create a new splicing branchpoint sequence that induces a partial mis-splicing of the pre-mRNA, leading to the introduction of a Premature Termination Codon. We characterized 5 accessions carrying this A-to-T substitution in intron seven and observed a complete correlation between this SNP and both a 10 to 20% level of the RPT5b pre-mRNA mis-splicing and the lack of ability to complement an rpt5a mutant phenotype.

ConclusionThe accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-10-158 contains supplementary material, which is available to authorized users.

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Autor: Anouchka Guyon-Debast - Alain Lécureuil - Sandrine Bonhomme - Philippe Guerche - Jean-Luc Gallois

Fuente: https://link.springer.com/



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