Characterization of alphasatellites associated with monopartite begomovirus-betasatellite complexes in Yunnan, ChinaReportar como inadecuado

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Virology Journal

, 7:178

First Online: 03 August 2010Received: 12 May 2010Accepted: 03 August 2010


BackgroundAlphasatellites are single-stranded molecules that are associated with monopartite begomovirus-betasatellite complexes.

ResultsAlphasatellites were identified in begomovirus-infected plant samples in Yunnan, China. All samples that contained alphasatellites also contained betasatellites, but only some samples that contained betasatellites contained alphasatellites. Thirty-three alphasatellites were sequenced, and they ranged from 1360 to 1376 nucleotides. All alphasatellites contain 3 conserved features: a single open reading frame Rep, a conserved hairpin structure, and an adenine-rich A-rich region. On the basis of the phylogenetic tree of the complete nucleotide sequences, the alphasatellites were divided into 3 types with one exception. Type 1 was associated with Tomato yellow leaf curl China virus TYLCCNV-Tomato yellow leaf curl China betasatellite TYLCCNB complex. Type 2 was associated with Tobacco curly shoot virus TbCSV-Tobacco curly shoot betasatellite TbCSB complex. Type 3 was associated with TbCSV-Ageratum yellow vein betasatellite AYVB complex. Within each type, nucleotide sequence identity ranged from 83.4 to 99.7%, while 63.4-81.3% identity was found between types. Mixed infections of alphasatellites associated with begomovirus-betasatellite complexes were documented.

ConclusionsOur results validate that alphasatellites are only associated with begomovirus-betasatellite complexes. Thirty-three sequenced alphasatellites isolated from Yunnan Province, China were divided into 3 types-each associated with a specific begomovirus-betasatellite complex. Mix-infections of alphasatellite molecules may not be unusual.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-7-178 contains supplementary material, which is available to authorized users.

Yan Xie, Peijun Wu contributed equally to this work.

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Autor: Yan Xie - Peijun Wu - Pei Liu - Huanran Gong - Xueping Zhou


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