Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virusReportar como inadecuado




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Virology Journal

, 7:241

First Online: 17 September 2010Received: 20 August 2010Accepted: 17 September 2010

Abstract

Duck virus enteritis DVE is an acute, contagious herpesvirus infection of ducks, geese, and swans, which has produced significant economic losses in domestic and wild waterfowl. With the purpose of decreasing economic losses in the commercial duck industry, studying the unknown glycoprotein K gK of DEV may be a new method for preferably preventing and curing this disease. So this is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay, the truncated glycoprotein K tgK has good antigenicity, also the antibody possesses high specificity and affinity. Meanwhile the rabbit anti-tgK polyclonal antibody has the potential to produce subunit vaccines and the functions of neutralizing DEV and anti-DEV infection because of its neutralization titer. Indirect immunofluorescent microscopy using the purified rabbit anti-tgK polyclonal antibody as diagnostic antibody was susceptive to detect a small quantity of antigen in tissues or cells. This approach also provides effective experimental technology for epidemiological investigation and retrospective diagnose of the preservative paraffin blocks.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-7-241 contains supplementary material, which is available to authorized users.

Shunchuan Zhang, Jun Xiang contributed equally to this work.

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Autor: Shunchuan Zhang - Jun Xiang - Anchun Cheng - Mingshu Wang - Xin Li - Lijuan Li - Xiwen Chen - Dekang Zhu - Qihui Luo - Xi

Fuente: https://link.springer.com/







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