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Proteome Science

, 8:52

First Online: 18 October 2010Received: 14 April 2010Accepted: 18 October 2010

Abstract

BackgroundUse of mobile phones has widely increased over the past decade. However, in spite of the extensive research, the question of potential health effects of the mobile phone radiation remains unanswered. We have earlier proposed, and applied, proteomics as a tool to study biological effects of the mobile phone radiation, using as a model human endothelial cell line EA.hy926. Exposure of EA.hy926 cells to 900 MHz GSM radiation has caused statistically significant changes in expression of numerous proteins. However, exposure of EA.hy926 cells to 1800 MHz GSM signal had only very small effect on cell proteome, as compared with 900 MHz GSM exposure. In the present study, using as model human primary endothelial cells, we have examined whether exposure to 1800 MHz GSM mobile phone radiation can affect cell proteome.

ResultsPrimary human umbilical vein endothelial cells and primary human brain microvascular endothelial cells were exposed for 1 hour to 1800 MHz GSM mobile phone radiation at an average specific absorption rate of 2.0 W-kg. The cells were harvested immediately after the exposure and the protein expression patterns of the sham-exposed and radiation-exposed cells were examined using two dimensional difference gel electrophoresis-based proteomics 2DE-DIGE. There were observed numerous differences between the proteomes of human umbilical vein endothelial cells and human brain microvascular endothelial cells both sham-exposed. These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone radiation did not cause any statistically significant changes in protein expression.

ConclusionsExposure of primary human endothelial cells to the mobile phone radiation, 1800 MHz GSM signal for 1 hour at an average specific absorption rate of 2.0 W-kg, does not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with our earlier study showing that the 1800 MHz GSM radiation exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926, as compared with the effect of 900 MHz GSM radiation.

List of Abbreviations2DEtwo-dimensional electrophoresis

BVABiological Variation Analysis module in DeCyder

CHAPS3-3-Cholamidopropyldimethylammonio-1-propanesulfonate

ddH2ODouble distilled water

DIGEDifference Gel Electrophoresis

DTTDithioreitol

EA.hy926Human endothelial cell line

EDAExtended Data Analysis module in DeCyder

FDRFalse discovery rate

GSMGlobal System for Mobile Communications

HBMECHuman Brain Microvascular Endothelial Cell

HUVECHuman Umbilical Vein Endothelial Cell

IAAIodoacetamide

IEFIsoelectric focusing

IPGImmobilized pH gradient

PAGEPolyacrylamide gel electrophoresis

PBSPhosphate buffered saline

pIIsoelectric point

PMSFphenylmethylsulphonyl fluoride

SARSpecific absorption rate

SDSSodium dodecyl sulfate

Tris-HClTrishydroxymethylaminomethane hydrochloride, Versene Chelating agent containing EDTA

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-8-52 contains supplementary material, which is available to authorized users.

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Autor: Reetta Nylund - Niels Kuster - Dariusz Leszczynski

Fuente: https://link.springer.com/







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