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The Protein Journal

, Volume 29, Issue 8, pp 538–544

First Online: 04 November 2010

Abstract

The importance of insulin-like growth factor-1 IGF-1 in coronary artery disease CAD due to wide range of its biological effects and its therapeutic potential, has already been described. Our aim was to evaluate possible influence of IGF-1 serum level changes on coronary atherosclerosis. In case of existence of such association our further aim was to verify and explain this phenomenon by examination of promoter P1 of IGF-1gene and receptor gene for IGF-1. The study was performed in 101 consecutive patients undergo for routine coronary angiography. Quantitative and qualitative assessment of coronary atherosclerosis was performed respectively by estimation of the number of culprit lesions in coronary arteries and by Gensini score calculation. IGF-1, IGFBP3 and plasma lipoproteins were measured in all patients. In addition, we evaluated DNA from 101 patients, isolated from blood cells, which was amplified by using PCR with sophisticated primers for P1 promoter of IGF-1 gene and IGF-1 receptor gene, then analyzed utilizing SSCP technique and automatically sequenced. We observed significant increase of serum IGF-1 levels in patients with -3 vessel disease- and with high score in Gensini scale when compared to those without any narrowing lesions in coronary arteries and 0 Gensini score in group with 3 vessel disease 215.0 ± 71.3 versuss 176.7 ± 34.2 ng-ml p = 0.04 and with high Gensini score 231.4 ± 59.3 versus 181.0 ± 37.8 ng-ml p = 0.01.We found different genotypes for five P1 promoter polymorphisms of IGF-1 gene RS35767, RS5742612, RS228837, RS11829693, RS17879774. There were no significant associations between the observed single nucleotide polymorphism SNP and coronary atherosclerosis nor with levels of circulating IGF-1. We found no structural polymorphism in receptor gene for IGF-1 nor in its extracellular domainexon 2–4 nor in internal domain exon 16–21. The effect of increased IGF-1 serum level in our study was probably independent from structural polymorphism in promoter P1 for IGF-1 or in receptor gene for IGF-1.

KeywordsIGF-1 Promoter P1 polymorphism Growth hormones CAD AbbreviationsApo A1Apoprotein A1

Apo B100Apoprotein B-100

BMIBody mass index

CADCoronary artery disease

DMDiabetes mellitus

GHGrowth hormone

HDL,LDLHigh-low density lipoproteins

IGFInsulin like growth factor

LpaLipoprotein a

MIMyocardial infarction

NtNucleotide

NYHANew York Heart Association- the clinical classification of advancing of heart failure

QCAQuantitative coronary angiography

SSCPSingle strand conformational polymorphism

TCTotal cholesterol

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Autor: Pawel Burchardt - Anna Gozdzicka-Jozefiak - Jakub Zurawski - Witold Nowak - Julia Durzynska - Rafał Link - Tomasz Grotowsk

Fuente: https://link.springer.com/







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