Use of an innovative T-tube maze assay and the proboscis extension response assay to assess sublethal effects of GM products and pesticides on learning capacity of the honey bee Apis mellifera L.Reportar como inadecuado




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Ecotoxicology

, Volume 19, Issue 8, pp 1612–1619

First Online: 26 September 2010Accepted: 10 September 2010

Abstract

Transgenic Cry1Ac+CpTI cotton CCRI41 is a promising cotton cultivar throughout China but side effects and especially sublethal effects of this transgenic cultivar on beneficial insects remain poorly studied. More specifically potential sublethal effects on behavioural traits of the honey bee Apis mellifera L. have not been formally assessed despite the importance of honey bees for pollination. The goal of our study was to assess potential effects of CCRI41 cotton pollen on visual and olfactory learning by honey bees. After a 7-day oral chronic exposure to honey mixed with either CCRI41 pollen, imidacloprid-treated conventional pollen used as positive sublethal control or conventional pollen control, learning performance was evaluated by the classical proboscis extension reflex PER procedure as well as a T-tube maze test. The latter assay was designed as a new device to assess potential side effects of pesticides on visual associative learning of honey bees. These two procedures were complementary because the former focused on olfactory learning while the latter was involved in visual learning based on visual orientation ability. Oral exposure to CCRI41 pollen did not affect learning capacities of honey bees in both the T-tube maze and PER tests. However, exposure to imidacloprid resulted in reduced visual learning capacities in T-tube maze evaluation and decreased olfactory learning performances measured with PER. The implications of these results are discussed in terms of risks of transgenic CCRI41 cotton crops for honey bees.

KeywordsSublethal effect Proboscis extension response Visual learning ability T-tube maze Cry1Ac CpTI Cotton Pollen Imidacloprid  Download fulltext PDF



Autor: Peng Han - Chang-Ying Niu - Chao-Liang Lei - Jin-Jie Cui - Nicolas Desneux

Fuente: https://link.springer.com/







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